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Research

Methylene blue (methylthioninium chloride) — living evidence map

The Atlas page holds the claims; this is how its evidence evolved.

Summary

Methylthioninium chloride (methylene blue) is the standard treatment for methemoglobinemia; in 185 cases treated over 24 years at a New York poison center, 98% improved, and adverse effects were reported in 4.9%. · pmid:40062661

Mechanism

Methylene blue can carry electrons in the mitochondrial chain directly from NADH to cytochrome c, and has limited neurodegeneration in animal models of stroke, Alzheimer's and Parkinson's disease. · pmid:28840449

Identity

PubChem CID 6099: Methylene Blue, C16H18ClN3S, 319.9 g/mol. · pubchem:6099

CAS registry number 61-73-4. · pubchem:6099

IUPAC name: [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride. · pubchem:6099

Where the evidence actually sits

Established50%
Preclinical only50%

Proportion of the 2 claims about Methylene blue (methylthioninium chloride) standing at each tier. Community volume is shown on the Consensus tab beside this and is never averaged into it.

Living evidence history

From Mitochondrial Function to Neuroprotection-an Emerging Role for Methylene Blue. (2018) · preclinicalEffectiveness and tolerability of methylthioninium chloride (methylene blue) for the treatment of methemoglobinemia: twenty-four years of experience at a single poison center. (2025) · established20182025

2 resolved citations, oldest to newest, coloured by tier. Retractions and contradictions land here as they are recorded.

Structure

No structure representation on record. PepJunkie draws only what an authoritative record supplies; for a peptide that is the residue sequence, and none has been curated for Methylene blue (methylthioninium chloride) yet.

Dose evidence, rung by rung

A studied human dose exists on file. Open the rung: the dose is the paper's, in its context, never PepJunkie's.

  1. Approved-label dose · a resolved regulatory document names a labelled dose

    nothing resolved on this rung

  2. Studied human doses · resolved human studies at the established tier
    • Effectiveness and tolerability of methylthioninium chloride (methylene blue) for the treatment of methemoglobinemia: twenty-four years of experience at a single poison center. · pmid 40062661 · 2025
  3. Observational evidence · resolved human data at the emerging tier: thin, or contested

    nothing resolved on this rung

  4. Community-reported protocols · protocol propositions from distinct members, counted once per human

    nothing resolved on this rung

  5. Preclinical evidence · resolved animal, cell or mechanistic work
    • From Mitochondrial Function to Neuroprotection-an Emerging Role for Methylene Blue. · pmid 28840449 · 2018

2 resolved citations, 0 unresolved (an unresolved citation lends nothing). Community volume is its own rung and is never averaged into the evidence rungs.