Edetate disodium (EDTA) is a chelating agent; in a monoclonal antibody formulation it mitigated both polysorbate 80 loss and methionine oxidation, proposed to act by chelating calcium and so reducing lipase activity.
preclinical — animal, cell or mechanistic work — not yet about people
every claim resolves to its source
All 2 citations resolve to their original source. · 0 posts naming it, 0 members counted once each.
Summary
Edetate disodium (EDTA) is a chelating agent; in a monoclonal antibody formulation it mitigated both polysorbate 80 loss and methionine oxidation, proposed to act by chelating calcium and so reducing lipase activity. · pmid:30502482
Mechanism
A study of three antibody proteins found residue-specific effects of EDTA and methionine on oxidation under iron and peroxide stress. · pmid:36130676
Proportion of the 2 claims about EDTA-2Na standing at each tier. Community volume is shown on the Consensus tab beside this and is never averaged into it.
Living evidence history
2 resolved citations, oldest to newest, coloured by tier. Retractions and contradictions land here as they are recorded.
Structure
No structure representation on record. PepJunkie draws only what an authoritative record supplies; for a peptide that is the residue sequence, and none has been curated for EDTA-2Na yet.
Dose evidence, rung by rung
No established safe human dose. Nothing on file establishes one. That is a statement about the evidence PepJunkie holds, not a promise about the molecule.
Approved-label dose· a resolved regulatory document names a labelled dose
nothing resolved on this rung
Studied human doses· resolved human studies at the established tier
nothing resolved on this rung
Observational evidence· resolved human data at the emerging tier: thin, or contested
nothing resolved on this rung
Community-reported protocols· protocol propositions from distinct members, counted once per human
nothing resolved on this rung
Preclinical evidence· resolved animal, cell or mechanistic work
Edetate Disodium as a Polysorbate Degradation and Monoclonal Antibody Oxidation Stabilizer. · pmid 30502482 · 2019
Residue-Specific Impact of EDTA and Methionine on Protein Oxidation in Biotherapeutics Formulations Using an Integrated Biotherapeutics Drug Product Development Workflow. · pmid 36130676 · 2023
2 resolved citations, 0 unresolved (an unresolved citation lends nothing). Community volume is its own rung and is never averaged into the evidence rungs.
Negative findings
failure is information
Nobody has filed a failure yet. Failures are information; this list is waiting for an honest one.