Free L-methionine is used as an antioxidant in protein formulations; above 20 mM it outperformed conventional antioxidants in limiting oxidation and aggregation of a high-concentration antibody.
established — replicated in humans, or settled by a regulator
every claim resolves to its source
All 4 citations resolve to their original source. · 0 posts naming it, 0 members counted once each.
Summary
Free L-methionine is used as an antioxidant in protein formulations; above 20 mM it outperformed conventional antioxidants in limiting oxidation and aggregation of a high-concentration antibody. · pmid:40978010
Methionine is a nutritionally indispensable, sulfur-containing amino acid: it supplies cysteine, and so glutathione, through the transsulfuration pathway, and is the body's main methyl donor through S-adenosylmethionine. · pmid:33000159
Mechanism
Its effect on oxidation differs by residue: a study of three antibody proteins mapped residue-specific protection by methionine and EDTA. · pmid:36130676
Restricting dietary methionine altered one-carbon metabolism and improved responses to chemotherapy or radiation in mouse cancer models, and produced similar metabolic changes in a controlled human feeding study. · pmid:31367041
Proportion of the 4 claims about L-Methionine standing at each tier. Community volume is shown on the Consensus tab beside this and is never averaged into it.
Living evidence history
4 resolved citations, oldest to newest, coloured by tier. Retractions and contradictions land here as they are recorded.
Structure
No structure representation on record. PepJunkie draws only what an authoritative record supplies; for a peptide that is the residue sequence, and none has been curated for L-Methionine yet.
Dose evidence, rung by rung
A studied human dose exists on file. Open the rung: the dose is the paper's, in its context, never PepJunkie's.
Approved-label dose· a resolved regulatory document names a labelled dose
nothing resolved on this rung
Studied human doses· resolved human studies at the established tier
Methionine Nutrition and Metabolism: Insights from Animal Studies to Inform Human Nutrition. · pmid 33000159 · 2020
Observational evidence· resolved human data at the emerging tier: thin, or contested
nothing resolved on this rung
Community-reported protocols· protocol propositions from distinct members, counted once per human
nothing resolved on this rung
Preclinical evidence· resolved animal, cell or mechanistic work
Dietary methionine influences therapy in mouse cancer models and alters human metabolism. · pmid 31367041 · 2019
Residue-Specific Impact of EDTA and Methionine on Protein Oxidation in Biotherapeutics Formulations Using an Integrated Biotherapeutics Drug Product Development Workflow. · pmid 36130676 · 2023
High-concentration L-methionine as a potent antioxidant for oxidation resistance and stability enhancement in high-concentration antibody therapeutics. · pmid 40978010 · 2025
4 resolved citations, 0 unresolved (an unresolved citation lends nothing). Community volume is its own rung and is never averaged into the evidence rungs.
Negative findings
failure is information
Nobody has filed a failure yet. Failures are information; this list is waiting for an honest one.