Tris HCl

Summary

Tris is a common biological buffer; stored at 70 C in a peptide formulation it degraded to liberate formaldehyde, which reacted with the peptide's tyrosine residue. · pmid:11536224

Identity

PubChem CID 93573: Tromethamine hydrochloride, C4H12ClNO3, 157.59 g/mol. · pubchem:93573

CAS registry number 1185-53-1. · pubchem:93573

IUPAC name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride. · pubchem:93573

Where the evidence actually sits

Proportion of the 1 claim about Tris HCl standing at each tier. Community volume is shown on the Consensus tab beside this and is never averaged into it.

Living evidence history

1 resolved citation, oldest to newest, coloured by tier. Retractions and contradictions land here as they are recorded.

Structure

No structure representation on record. PepJunkie draws only what an authoritative record supplies; for a peptide that is the residue sequence, and none has been curated for Tris HCl yet.

Dose evidence, rung by rung

No established safe human dose. Nothing on file establishes one. That is a statement about the evidence PepJunkie holds, not a promise about the molecule.

1. Approved-label dose · a resolved regulatory document names a labelled dose

   nothing resolved on this rung

2. Studied human doses · resolved human studies at the established tier

   nothing resolved on this rung

3. Observational evidence · resolved human data at the emerging tier: thin, or contested

   nothing resolved on this rung

4. Community-reported protocols · protocol propositions from distinct members, counted once per human

   nothing resolved on this rung

5. Preclinical evidence · resolved animal, cell or mechanistic work
   • Formaldehyde production by Tris buffer in peptide formulations at elevated temperature. · pmid 11536224 · 2001

1 resolved citation, 0 unresolved (an unresolved citation lends nothing). Community volume is its own rung and is never averaged into the evidence rungs.